Triptolide inhibits activity of human cervical microvascular endothelial cells

VernacularTitle:雷公藤甲素对人子宫颈微血管内皮细胞活性的抑制
Author: ZHANG Yali ¹ ; DIAO Yunyun ¹ ; ZHANG Chunze ²
Author Information

1. Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Hebei North College
2. Department ofAnorectal Surgery, Tianjin Peoples Hospital
Publication Type:Journal Article
Keywords: triptolide; vasculogenesis; endothelial cells; VEGF
From: Chinese Journal of Cancer Biotherapy 2019;26(2):196-199
CountryChina
Language:Chinese
Abstract: Objective: To investigate the inhibitory effect of Triptolide on vasculogenesis of human cervical microvascular endothelial cells, and to explore the mechanism. Methods: Human cervical microvascular endothelial cells (HCerMECs) were used as research subject, and treated with different concentrations of Triptolide (0, 5, 10, 20 and 40 ng/ml) i n v i t r o . The effect of Triptolide on cell proliferation was determined by CCK-8, the cell migration ability was detected by Transwell assay while the expression of vascular endothelial growth factor (VEGF) was examined by western blotting. Results: Triptolide inhibited the proliferation and migration of HCerMECs in a dose-dependent manner ( P <0.05). In addition, Triptolide could inhibit the expression of VEGF in HCerMECs in a concentration-dependent manner. Conclusions: Triptolide could inhibit the proliferation and migration activity of HCerMECs which is related with the suppression of VEGF expression.
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