Screening and verification of key Hub genes in esophageal squamous cell carcinoma based on bioinformatics analysis
10.3872/j.issn.1007-385x.2019.02.005
- VernacularTitle:基于生物信息学分析的食管鳞状细胞癌关键枢纽基因的筛选及验证
- Author:
GUO Yanli
;
LIANG Xiaoliang
;
KUANG Gang
;
WU Xuan
;
KANG Xiaoliang
;
DONG Zhiming
;
SHEN Supeng
;
LIANG Jia
;
GUO Wei
- Publication Type:Journal Article
- Keywords:
esophageal squamous cell carcinoma;
bioinformatics analysis;
Hub genes;
GSE100942
- From:
Chinese Journal of Cancer Biotherapy
2019;26(2):166-172
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To screen the Hub genes associated with the occurrence and development of esophageal squamous cell carcinoma (ESCC) and to analyze their biological functions by using various bioinformatics analysis tools. Methods: ESCC chip profile GSE100942 from GEO database was used as study subject; GEO2R tool was used to analyze the data and to screen the differentially expressed genes (DEGs), and the bioinformatics tools (DAVID, String, Cytoscape) were further used to construct protein-protein interaction (PPI) network and identify the key Hub genes. GO and KEGG were used for the biological function enrichment analysis. In the meanwhile, MiRDB was applied to identify the miRNAs that might regulate Hub genes and to construct Hub gene–miRNA network. Importantly, the expression of DEGs and the patient survival were verified by the GEPIA analysis tool. Results: By analyzing GSE100942 database, a total of 1229 DEGs with difference of 2 times and 223 DEGs with difference of 4 times were screened out. In addition, 20 Hub genes, which were all up-regulated in ESCC tissues, were also identified. The functional enrichment analysis showed that these DEGs were mainly enriched in cancer related pathways and involved in cell division and mitotic nuclear division. Among those 20 Hub genes, DLGAP5, BUB1B, TPX2, TTK, CDC20, CCNB2, AURKA and DEPDC1 were identified as 8 key Hub genes that related with ESCC, and involved in many important biological processes, such as cell proliferation, cell cycle and signal pathway. Five Hub genes, CEP55, ECT2, NEK2, DEPDC1 and NUSAP1, were identified to be highly regulated by the miRNA regulatory network. Conclusion: Microarray combined with bioinformatics can effectively analyze the DEGs associated with the occurrence and development of ESCC. The identification of the 20 Hub genes and the 8 key Hub genes can provide theoretical guidance for further research on the molecular mechanism and molecular marker screening of ESCC.
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- Full text:20190205.pdf
