Distribution of CD103+CD8+T cell infiltration in colorectal cancer tissues and its clinical significance
10.3872/j.issn.1007-385x.2019.01.009
- VernacularTitle:CD103+CD8+T细胞在结直肠癌组织中的浸润分布及其临床意义
- Author:
LU Huimin
1
,
2
;
WANG Yan
1
,
2
;
CHEN Lujun
3
;
JIANG Jingting
1
,
2
;
LU Binfeng
1
,
4
,
5
Author Information
1. (1a.Medical College
2. 1b.Tumor Biological Treatment Center, the Third Affiliated Hospital of Soochow University, Engineering Technology Research Center for Tumor Immunotherapy of Jiangsu Province, Institute of Cell Therapy of Soochow University
3. Tumor Biological Treatment Center, the Third Affiliated Hospital of Soochow University, Engineering Technology Research Center for Tumor Immunotherapy of Jiangsu Province, Institute of Cell Therapy of Soochow University
4. 1b.Tumor Biological Treatment Center, the Third Affiliated Hospital of Soochow University, Engineering Technology Research Center for Tumor Immunotherapy of Jiangsu Province, Institute of Cell Therapy of Soochow University, Changzhou 213003, Jiangsu, China
5. 2.Department of Immunology, School of Medicine,University of Pittsburgh, Pittsburgh PA15213, United States
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
CD103+CD8+T cell;
tissue microarray;
prognosis
- From:
Chinese Journal of Cancer Biotherapy
2019;26(1):50-57
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the degree and distribution of tissue-resident CD8+ T cell (CD103+CD8+T cells) infiltration in colorectal cancer (CRC) tissues, and to analyze its relationship to patients’clinicopathological features and prognosis. Methods: Tissue chips of 88 cases of colon cancer tissues (No.HColA180Su14) and 77 cases of rectal cancer tissues (No. HRec-Ade180Sur-03) were obtained from Shanghai Outdo Biotech Co.,Ltd. Immunofluorescence staining was performed to examine the infiltration pattern and degree of CD103+CD8+T cells in the collected CRC tissues and their para-cancerous tissues. Wilcoxon rank test was used to compare CD103+CD8+T cell infiltration degree in CRC tissues and the para-cancerous tissues. Chi-square test was used to analyze the relationship between CD103+CD8+T cell infiltration in CRC and patients’clinicopathological features. Kaplan-Meier survival analysis was conducted to explore the correlation between CD103+CD8+T cell infiltration and patients’prognosis. Cox model was applied to analyze the correlation between different clinical parameters and patients’prognosis. Results: CD103+CD8+T cell infiltration presented no signifi
·cant differences between CRC and para-cancer tissues (P>0.05). Patients with distant metastasis had significantly lower CD103+CD8+T cell infiltration rate than patients without distant metastasis (P<0.01). There was no significant correlation between the infiltration of CD103+CD8+T cells and other clinicopathological features (P>0.05). Kaplan-Meier survival analysis showed that the overall survival (OS) of patients with high CD103+CD8+T cell infiltration was significantly longer than that of the patients with low infiltration (54.42% vs 25.00%, P<0.05). Multivariate Cox model analysis indicated that pathological grade (P<0.01) and high CD103+CD8+T cell infiltration (P<0.05) were independent prognostic factors for CRC. Conclusion： ：CD103+CD8+T cell infiltration in CRC is associated with patients’prognosis, suggesting that CD103+CD8+T cell plays an important role in the initiation and development of CRC.
- Full text:20190109.pdf
