Biocompatibility of bone marrow mesenchymal cells on polyetheretherketone and titanium surfaces in vitro
10.12016/j.issn.2096-1456.2020.02.002
- Author:
LIAN Keqian
1
;
ZHANG Xin
2
;
ZHOU Jieyu
1
;
LIAO Yanfen
1
;
SI Shanshan
3
Author Information
1. Department of Stomatology, The First Affiliated Hospital, Sun Yat-sen University
2. Department of Implantology and Prosthodontics, Stomatological Hospital, Southern Medical University
3. Department of General Emergency, Stomatological Hospital, Southern Medical University
- Publication Type:Journal Article
- Keywords:
bone marrow mesenchymal cells;
polyetheretherketone;
titanium;
biocompatibility;
dental implants;
adhesion;
proliferation;
osteogenesis differentiation;
alkaline phosphatase;
mineralized nodule
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2020;28(2):73-78
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the in vitro biocompatibility of bone marrow mesenchymal cells on polyetheretherketone (PEEK) and titanium (Ti) surfaces.
Methods :PEEK and Ti foils with thicknesses of 1 mm and diameters of 10 mm were prepared. First, bone marrow mesenchymal cells were separated and purified by the whole bone marrow adherent culture method in vitro. Then, osteogenesis-induced bone marrow mesenchymal cells were cultivated on the surfaces of the PEEK and Ti foils. Scanning electron microscopy (SEM), the Alamar Blue test, an alkaline phosphatase (ALP) kit and Alizarin Red staining were used to analyze calcium nodules and compare the adhesion, proliferation and osteogenic differentiation ability of bone marrow mesenchymal cells on the surfaces of the PEEK and Ti foils.
Results : ① The morphology of the bone marrow mesenchymal cells cultured on the PEEK and Ti foils at 1 h, 4 h and 24 h showed no significant differences. ② In the 1 h, 3 h, 1 d and 3 d cultures of the bone marrow mesenchymal cells inoculated on the surfaces of the foils, the number of living cells in the PEEK group was greater than that in the Ti group (P < 0.05). ③ In the 7 d and 14 d osteogenesis-induced cultures of the inoculated bone marrow mesenchymal cells, the ALP activity of the PEEK group cells was significantly greater than that of the Ti group cells (P < 0.05). ④ Semiquantitative analysis after Alizarin Red staining showed that the mineralization degree of the bone marrow mesenchymal cells induced by osteoblasts was greater in the PEEK group than in the Ti group (P < 0.05).
Conclusion:PEEK has better in vitro biocompatibility than Ti and can better promote cell adhesion, proliferation and osteogenic differentiation compared with Ti, and so it is expected to become a new dental implant material.
- Full text:骨髓间充质细胞在聚醚醚酮片和纯钛片表面的体外生物相容性比较.pdf
