Optimization of chromatographic conditions for the determination of clenbuterol hydrochloride residues in animal edible product
10.19485/j.cnki.issn2096-5087.2018.06.007
- VernacularTitle:动物性食品中盐酸克仑特罗残留检测色谱条件优化
- Author:
Yi-Yao CAO
1
;
Shun-Fei YU
;
Zhi-Qiang XUAN
;
Yao-Xian ZHAO
;
Xin-Xing LI
;
Shou-Ming WU
;
San-Hu ZHAO
;
Ping LIU
Author Information
1. 浙江省疾病预防控制中心
- Keywords:
HPLC;
Animal edible product;
Clenbuterol hydrochloride
- From:
Journal of Preventive Medicine
2018;30(6):570-573
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high performance liquid chromatography (HPLC) through the optimization of the chromatographic conditions, which can detect the contents of clenbuterol hydrochloride (CL) residues in animal edible product in a large quantity. Methods The animal edible product were extracted by perchloric acid, and then impurities were removed by liquid-liquid extraction (LLE) which used ethyl acetate- isopropanol. After the organic phase was concentrated, C18 column (150 mm×4.6 mm, 5 μm) was used to separate CL. Mobile phase were methanol-sodium dihydrogen phosphate, and then determined by HPLC. Results A good linear response was obtained over the range of 0.2-10.0 μg/mL with the correlation coefficient (r) 0.99984. The method determination limit was 0.15 μg/kg which was lower than the National standard method 0.5μg/kg. The retention time of the CL was 6.51 min, the chromatographic peak was good. The recovery rates spiked with standards 1.6-12 μg were 92.86％-100.93％, which was higher than National standard method (89.79％-92.36％) . The precision of intra-day and inter-day were both under 5％, which lower than National standard. Conclusion The optimized chromatographic conditions are suitable for the large quantity determination of clenbuterol hydrochloride in animal edible product.
