A study on the effect of Cucurbitacin B on the inflammatory reaction of murine alveolar macrophages induced by lipopolysaccharide
10.19485/j.cnki.issn1007-0931.2017.07.007
- VernacularTitle:葫芦素B对脂多糖诱导小鼠肺泡巨噬细胞炎症反应的影响
- Author:
Kang ZHOU
1
;
Yong-Wei ZHANG
;
Xin-Jian LU
;
Chun-Fang MA
Author Information
1. 浙江省新华医院检验科
- Keywords:
Cucurbitacin B;
Lipopolysaccharide;
Macrophage-inflammatory cytokine
- From:
Journal of Preventive Medicine
2017;29(7):680-683,688
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-inflammatory effect of Cucurbitacin B on the inflammatory reaction of murine alveolar macrophages induced by lipopolysaccharide and its molecular mechanism. Methods The alveolar macrophages were randomly divided into five groups: blank group, LPS group, different concentrations of Cucurbitacin B (1μM, 2.5μM, 5μM)+) +LPS groups. All group cells were pretreated with different concentrations for 2 h, and followed by 1μg/mL LPS. The protein level of TNF-α, IL-1β, IL-6 and PGE2 were detected by ELISA assay, and the NO was detected by Griess methods, and its upstream protein i-NOS,COX-2, Nrf2 and HO-1 were detected by Western-blot. Results The secretion of TNF-α, IL-1β, IL-6 and PGE2 in LPS group were increased significantly than blank groups, while Cucurbitacin B suppressed its protein level inadose-dependent manner. The nuclear erythroid related factor 2 and antioxidant gene were activated while Cucurbitacin B was added into alveolar macrophages in adose-dependent manner. Conclusion Cucurbitacin B can inhibit the extracellar secretion of inflammatory cytokines production, and its molecular mechanism could be due to the promoting Nrf2 translocation to the nucleus, activating the expression of HO-1 gene, reducing the expression of iNOS and COX-2, and thereby alleviating the inflammatory reaction.
