Antidepressant effect of flavonoids extracted from Apocynum venetum leaves and expression of S1 00 A1 0 in rat model of depression
- VernacularTitle:罗布麻叶总黄酮提取物对抑郁大鼠的作用和对S100 A10表达的影响
- Author:
Jia WANG
1
;
Jian XIE
;
Yue-Ming CHEN
Author Information
1. 杭州市第一人民医院
- Keywords:
Apocynum venetum;
Depression;
S1 00A1 0
- From:
Journal of Preventive Medicine
2016;28(6):574-577
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the antidepressant effect and underlying mechanism of flavonoids extracted from Apocynum venetum leaves (AVL -extracts)in rat depression model.Methods The rats were randomly divided into seven parts including the control group,depression model group,low dose AVL-extracts prevention group (40 mg/kg),high dose AVL-extracts prevention group (80 mg/kg),low dose AVL-extracts treatment group (40 mg/kg),high dose AVL-extracts treatment group (80 mg/kg ) and fluoxetine treatment group, respectively. The rat depression model was established by the method of chronic unpredictable mild stress (CUMS ).The open field test was used to evaluate the autonomic activities.The relative expression levels of S1 00A1 0 mRNA were detected by Real-time PCR.Results The open field activities decreased significantly in the depression model group including horizontal movements and vertical movements with comparison to the normal group (P <0. 05 ).The autonomic activities increased significantly after preventive administration of high dose AVL-extracts (P<0. 05 ).The antidepressant effect of AVL-extracts was similar to that of fluoxetine in increasing the autonomic activities.The S1 00A1 0 mRNA expressed in the depressive group were higher than in the control group (P<0. 05 ),and the expression levels were significantly decreased after fluoxetine and AVL-extracts administration (P <0. 05 ).The high dose AVL -extracts administration group and fluoxetine group showed no significant difference in the expression levels of S1 00A1 0 mRNA when compared to the control group (P>0. 05 ),whereas the levels of those in low dose AVL-extracts administration groups were significantly lower than those in the control group (P<0. 05 ).Conclusion The study suggests that AVL -extracts have antidepressant effects on rat depression model.The underling mechanism of AVL-extracts antidepressant effect may be associated with regulation of S1 00A1 0 mRNA expression.
