An analysis on plasma microRNA profiling and function among population exposed to asbestos
- VernacularTitle:石棉暴露人群血浆中差异 microRNA筛选及功能分析
- Author:
Xian-Hong YIN
1
;
Hai-Ling XIA
;
Zhao-Qiang JIANG
;
Min YU
;
Wei WU
;
Min ZHANG
;
Li-Jin ZHU
;
Jun-Qiang CHEN
;
Yun XIAO
;
Shi-Bo YING
;
Jian-Lin LOU
;
Xing ZHANG
Author Information
1. 浙江省医学科学院职业病防治研究所
- Keywords:
Asbestos exposure;
Plasma;
MicroRNA;
Target gene
- From:
Journal of Preventive Medicine
2016;(3):221-225
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of asbestos exposure on plasma miRNA expression.Methods Plasma samples were collected from control group and asbestos -exposed group (time of exposure >10 years)and three samples from each group were selected to detect differentially expressed miRNA using LC Sciences miRNA Microarray -Single.The target genes of differential miRNA were predicted by three kinds of online software,Target Scan,miRanda and PicTar.GO term enrichment and KEGG pathways were analyzed.Results The results of microarray indicated that there were 40 differential miRNA expression between exposed and control groups(P <0.05),and the signal value of 9 differential miRNA exceeded 500.After analyzing signal pathways of target genes of 5 miRNA,of which the signal values were over 500,these target genes were found mainly involved in pathways associated with cancer and metabolism,including potential function targets of FAS,TP53 and FGFR3.Conclusion Asbestos exposure can result in differentially expressed miRNA in the plasma from workers occupationally exposed to asbestos and the target genes of these miRNA may play important roles in the pathways of cancer.However,the mechanism of these miRNA in asbestos -related diseases needs to be further studied in the future.
