A study on the development of a triplex real -time RT -PCR for simultaneous detection of enteroviruses and differentiation of EV 71 and CA 16
- VernacularTitle:EV71、CA16和肠道病毒通用型三重荧光RT －PCR 检测技术的建立
- Author:
Chang-Ping XU
1
;
Chun-Hong ZHENG
;
Ju-Ying YAN
;
Qiong GE
;
Li-Ming GONG
;
Jiang-Feng GUO
Author Information
1. 浙江理工大学生命科学学院
- Keywords:
Hand,foot and mouth disease;
EV 71;
CA 16;
Real -time RT -PCR
- From:
Journal of Preventive Medicine
2016;(3):217-220
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a multiplex real -time RT -PCR assay for simultaneous detection of enteroviruses and differentiation of EV71 and CA16.Methods Specific primers and probes were designed for enteroviruses,EV71 and CA16.The probes labeled with various fluorescent reporter dyes,and a triplex real -time RT -PCR technique was developed to simultaneously detect these viruses.A total of 91 clinical specimens with suspected HFMD were analyzed by this method.Results This assay could simultaneously detect enterovirus and differentiation of EV71 and CA16,and the sensitivity of the assay was up to 0.1 TCID50 /mL,and only need 2 to 3 hours for completing the detection.A total of 91 clinical specimens were detected by this assay in 28 of the 91(30.77%)specimens contained EV71,9 of the 91(9.89%) contained CA16,and 5 of the 91 (5.49%)contained other enteroviruses.Conclusion This assay would be a useful molecular diagnostic tool for large -scale screening of clinical samples,especially at the peroid of HFMD outbreaks.
