A study on the application of MPB64 antigen detection of immune colloidal gold method for identification of Mycobacterium
- VernacularTitle:MPB64免疫胶体金检测法在分枝杆菌菌种鉴定中的应用
- Author:
Ai-Zhen PAN
1
;
Bei-Bei WU
;
Zheng-Wei LIU
;
Lin ZHENG
;
Xiao-Meng WANG
Author Information
1. 浙江省疾病预防控制中心
- Keywords:
Immune colloidal gold method;
Mycobacterium;
Species identification
- From:
Journal of Preventive Medicine
2015;(7):691-694
- CountryChina
- Language:Chinese
-
Abstract:
Objective To know the application of MPB64 antigen detection of immune colloidal gold method for identification of Mycobacterium.Methods Taking the PCR amplified sequencing method as the 'gold standard',a total of 418 clinical isolated mycobacterium tuberculosis and 2 standard strains were tested by immune colloidal gold method,and was compared with the traditional method of PNB/TCH.Results Compared with sequencing method,the coincidence rate of immune colloidal gold method and PNB/TCH identification test was 95. 93%and 87. 08%respectively.The sensitivity, specificity,positive predictive value (PPV ) and negative predictive value (NPV ) of immune colloidal gold method identification were 88. 41%,99. 64%,99. 19% and 94. 58%respectively.It costs MPB64 immune colloidal gold method 15 minutes to carry out the results,less than 28 days of the traditional method,as well as 3 days of sequencing method. Conclusion Compared with PNB/TCH method,MPB64 immune antigen colloidal gold method has higher specificity and sensitivity,and could be applied in identification of Mycobacterium.
