A study on the mechanism of biofilm formation of Pseudomonas aeruginosa and its relationship with drug resistance
- VernacularTitle:铜绿假单胞菌生物膜形成机制及耐药相关性
- Author:
Jian SHEN
1
;
Chen FENG
;
Yun-Zhao LIN
;
Wen-Hua ZHENG
;
Hua GU
;
Jin-Qin JIANG
;
Lei FENG
Author Information
1. 浙江医学高等专科学校
- Keywords:
pseudomonas aeruginosa;
Biofilm;
planktonic state;
Drug resistance
- From:
Journal of Preventive Medicine
2015;(1):40-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare antibiotic resistance of pseudomonas aeruginosa in biofilms and in planktonic state,so as to provide practical guidance and theoretical support for clinical treatment of drug. Methods pseudomonas aeruginosa were divided into control culture group( group A),medium group of biological membrane after culture( group B) and the biofilm culturing silica film adhesion group( group C). Three groups were observed by silver staining,extracellular sugar staining and scanning of electron microscope. These groups were cultured with different antibiotic medium,and the situation of bacterial resistance was observed by the method of k-B. Results There was no significant difference in drug resistance and minimum inhibitory concentration(MIC)between group A and B(p >0. 01). Compared with group A, group C showed that their drug resistance on meropenem,cefepime,ceftazidime,ceftriaxone,amoxicillin and levofloxacin were much higher(p<0. 01). The minimal biofilm eradication concentration(MBEC)of group C was about 100 times than that of group A and group B. Conclusion The assay of establishment of pseudomonas aeruginosa biofilm and the methods observed by silver stainingin vitro is convenient and feasible. At the same time,pseudomonas aeruginosa in biofilm exhibits far more resistance to antimicrobial than its planctonic counterparts does.
