A study on application of BacT/ALERT 3D liquid culture technology on tuberculosis drug resistance detection
- VernacularTitle:BacT／ALERT 3D 技术在结核杆菌耐药检测中的应用研究
- Author:
Wen-Zhong HUANG
1
;
Ping-Ping WANG
;
Hong-Zhao WU
;
Yan-Zhen ZHOU
;
Zhen-Bao HU
Author Information
1. 浦江县疾病预防控制中心
- Keywords:
Mycobacterium tuberculosis;
BacT/ALERT 3D liquid culture technology;
L -J proportion method;
Drug resistance
- From:
Journal of Preventive Medicine
2014;(8):764-767
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the BacT/ALERT 3D liquid culture technology on the detection of drug resistance of Mycobacterium tuberculosis(MTB)and to compare the difference between this technology and Lowenstein -Jensen (L -J) proportion method.Methods BacT/ALERT 3D liquid culture technology and L -J proportion technology were applied to detect the drug resistance of tuberculosis from the positive cultures of 219 solid culture samples.Results The average detection time of BacT/ALERT 3D method was 8.02 ±3.85 d,which was about 20 days shorter than that of L -J proportion method.60 drug resistance strains were found using BacT/ALERT 3D technology,While 79 drug resistance strains were found using L -J proportion technology.There showed no significant difference (P >0.05).The compliance rate of BacT/ALERT 3D method and L -J proportion method on the anti -tuberculosis drugs INH,RFP,EMB and SMwas 95.43%,92.69%,95.43% and 92.24% respectively.Conclusion BacT/ALERT 3D liquid culture technology could detect drug resistant TB strains rapidly with high concordance with the results of L -J proportion method on anti -tuberculosis drugs.
