A study on genotyping of mycobacterium tuberculosis by variable number tandem repeat analysis method
- VernacularTitle:VNTR位点对结核分枝杆菌基因分型的研究
- Author:
Si-Chao ZHANG
1
;
Zheng-Quan DONG
;
Zhong-Rong YANG
Author Information
1. 湖州市疾病预防控制中心
- Keywords:
Mycobacterium tuberculosis;
Genotyping;
Tandem repeat sequences
- From:
Journal of Preventive Medicine
2014;(3):221-223,228
- CountryChina
- Language:Chinese
-
Abstract:
Objective To preliminarily evaluate the ability of 15 -loci variable number tandem repeats on the genotyping of clinical isolates of mycobacterium tuberculosis,and explore the suitable loci for genotyping of Mycobacterium tuberculosis in Huzhou City.Methods From 201 1 January to 201 1 April,the clinical isolates of mycobacterium tuberculosis were selected from Huzhou area to test the polymorphism of every loci by using MLVA detection method and analyze hunter-gaston discriminatory index of individual locus as well as combinations of 15 locus.Results There were two locus of Mtub-21 and Mtub-39 which had better degree of polymorphism;seven locus of ETR-A,ETR-C,MIRU-10,MIRU-23,MIRU-27,MIRU-40 and Mtub-30 had moderate degree of polymorphism;six locus of ETR-B,ETR-D,ETR-E,MIRU-16,MIRU-26 and MIRU-39 had poor degree of polymorphism.By calculation,the result showed that HGDI was 0.99 by using 15 locus to genotype,which indicated the ability to distinguish the genotype was good.Conclusion We will continue to look for other rich VNTR polymorphism locus to fit the local region fast genotyping of mycobacterium tuberculosis for higher resolution and cost effectiveness of mycobacterium tuberculosis control and prevention.
