The effect evaluation on the application of BacT/ALERT 3 D technique for the fast cultivation of mycobacterium tuberculosis
- VernacularTitle:BacT／ALERT 3 D检测技术应用于结核分枝杆菌快速培养的效果评价
- Author:
Wen-Zhong HUANG
1
;
Ping-Ping WANG
;
Yan-Zhen ZHOU
;
Hong-Zhao WU
;
Zhen-Bao HU
Author Information
1. 浦江县疾病预防控制中心
- Keywords:
Mycobacterium tuberculosis;
BacT/ALERT 3D;
L-J solid culture mediums;
Fast cultivation
- From:
Journal of Preventive Medicine
2014;(3):217-220
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the BacT/ALERT 3D liquid rapid culture system for the rapid detection of mycobacterium tuberculosis(MTB)and early diagnosis of pulmonary tuberculosis.Methods BacT/ALERT 3D liquid culture mediums and lowenstein-jensen(L-J)solid culture mediums were applied to detect Mycobacterium tuberculosis sputum specimens respectively.Analysis of detection results and detection time were also performed.Results Average positive days of BacT/ALERT 3D liquid culture medium was(13 ±2.05)days,which was shorter than that of L-J solid culture mediums(34.7 ±4.76 )d (P<0.05 ).Compared to the L -J solid culture mediums,BacT/ALERT 3D liquid culture medium had higher positive rate for 59 patients whose sputum smear test was positive,and the positive rate were 71.18%(42/59)and 67.80%(40/59)respectively(P<0.05).BacT/ALERT 3D liquid culture medium had higher positive rate than L-J solid culture mediums for 106 patients whose sputum smear test was negative,and the positive rate were 39.62%(42/106)and 26.42%(28/106)respectively(P<0.05).Conclusion Compared to the traditional L-J solid culture system,BacT/ALERT 3D liquid culture system can shorten detection time and improve the positive detection rate of MTB in specimens with low concentration.
