Impact of 1.8GHz Radiofrequency Electromagnetic Fields on Gene Expression Profiles in Rat Neurons
10.3969/j.issn.1007-0931.2009.07.002
- VernacularTitle:1.8 GHz射频电磁场对大鼠神经元基因表达谱的影响
- Author:
Shu-Zhi ZHANG
1
;
Geng-Dong YAO
;
De-Qiang LU
;
Huai CHIANG
;
Zheng-Ping XU
Author Information
1. 浙江省医学科学院
- Keywords:
Radiofrequency electromagnetic fields;
Neuron;
Gene chip;
Gene expression profile
- From:
Journal of Preventive Medicine
2009;21(7):3-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of gene expression in rat neurons induced by 1.8 GHz radiofrequency electromagnetic fields (RF EMF) and to screen RF EMF-responsive genes. Methods The rat primary cultured neuronal cells were divided into two groups, the radiation group and control group, from which the total RNA was extracted immediately and purified after intermittently (5min on/10min off) exposed or U34 array was applied to detect the changes of gene expression in rat neurons. Results Among 1200 candidate genes, 24 up-regulated and 10 down-regulated genes which are associated with multiple cellular functions (cytoskeleton, signal transduction pathway, metabolism, etc.) after functional classification were found by using Affymetrix microarray suite software 5.0. Although the changes in gene expression were less than 2 folds, they had statistical significance (P<0.01). Conclusion RF radiation of 1.8GHz induce the changes of many genes transcription in rat neurons, some of which indicate the negative effects of RF radiation on neurons.
