Changes of macrophages phenotype markers in fibrous capsules around silicone implants
10.3760/cma.j.issn.1671-0290.2019.02.001
- VernacularTitle:大鼠硅胶植入物周围纤维包膜中巨噬细胞表型的变化
- Author:
Jianke DING
1
;
Lei LEI
;
Zhou YU
;
Yingjun SU
;
Xianjie MA
Author Information
1. 空军军医大学西京医院整形外科
- Keywords:
Macrophages;
Silica gel;
Prosthesis implantation;
Implant capsular contracture;
Rats
- From:
Chinese Journal of Medical Aesthetics and Cosmetology
2019;25(2):81-85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the temporal distribution of macrophage and its phenotype markers in fibrous capsules around silicone implants.Methods Thirty rats were randomly divided into five groups:days 1,3,7,14 and 35.Silicone prostheses (10 ml) were implanted subcutaneously into backs of rats.On each indicated day,the tissue specimens were collected,fixed in 4％ paraformaldehyde for 24 hours and embedded in paraffin.Immunofluorescence was used to detect temporal distribution of M1/M2 macrophages.Results The number of CD68+ macrophages at day 1 (65.8±12.9) was smaller than that at day 3 (102.8±14.5,P＜0.05) and day 7 (116.8±14.2,P＜0.05);and the number of CD68+ macrophages at day 7 was larger than that at day 14 (56.8±12.9,P＜0.05) and day 35 (21.40±6.35,P＜0.05);the proportion of iNOS+ CD68+ M1 cells at day 1 and day 3 was 0.48±0.13,0.60±0.13,respectively,and they were higher than that at day 7 (0.21±0.03,P＜0.05),day 14 (0.21±0.03,P＜0.05) and day 35 (0.17±0.04,P＜0.05);the proportions of CD206+ CD68+ M2 cells at day 1,day 3,day 7,day 14,day 35 were 0.70±0.06,0.60±0.07,0.70±0.08,0.67±0.02 and 0.60±0.06,respectively.Conclusions After the implantation of silicone prostheses,M1 cells increase in early stages and M2 cells maintain in high level throughout the experiment period.
