Application of metagenomic next generation sequencing in infectious diseases

VernacularTitle:宏基因组测序在感染性疾病中的应用与反思
Author: Mingyuan BI ¹ ; Chunfu WANG ; Jianqi LIAN ; Yongtao SUN
Author Information

1. 空军军医大学第二附属医院传染科
Keywords: Next generation sequencing; Metagenomic next generation sequencing; Infectious diseases; Application
From: Chinese Journal of Clinical Infectious Diseases 2019;12(5):379-384
CountryChina
Language:Chinese
Abstract: Almost all pathogens have DNA or RNA genomes.Theoretically, genomes of pathogens can be effectively detected by sequencing technology , it would be of value for etiological diagnosis of infectious diseases.The next generation sequencing ( NGS) emerged in 2005, the advantages of low cost , high efficiency and high accuracy make it a favorable technical platform for clinical identification of microorganisms.As a new sequencing technique , metagenomic next generation sequencing ( mNGS) has great potential in improving the accuracy and efficiency of pathogenic diagnosis , especially in the areas where conventional diagnostic methods have limitations.This article introduces the common platforms and principle of the high throughput sequencing , reviews the clinical applications and the advantages of mNGS in identification of different pathogenic microorganisms , and reflects on its limitations and seeks possible solutions.