A proteoglycan-induced spondyloarthritis mouse model and the therapeutic effect of CCN1 monoclonal antibody
10.3760/cma.j.issn.1007-7480.2019.08.003
- VernacularTitle:软骨蛋白聚糖诱导的脊柱关节炎小鼠模型的建立及富含半胱氨酸蛋白61单克隆抗体治疗效应初探
- Author:
Jiajie LI
1
;
Huidan LI
;
Jie ZHANG
;
Tianhang ZHAI
;
Rongfen HUO
;
Baihua SHEN
;
Weiwei XIN
;
Ting LI
;
Shuang YE
;
Ningli LI
Author Information
1. 上海交通大学医学院附属仁济医院南院风湿科 201112
- Keywords:
Spondylitis;
Disease;
Models,animal;
Inflammation;
Osteogenesis
- From:
Chinese Journal of Rheumatology
2019;23(8):513-517,后插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up a mouse model of spondyloarthritis,analyzethe clinical phenotype,radiographic and pathological features,and investigate the therapeutic effect of cysteine-rich 61 (CCN1) monoclonal antibody in spondyloarthritis mouse model.Methods Proteoglycan from bovine nasal septum was used for immunization of 14-16 week old female BALB/c mice.CCN1 monoclonal antibody 093G9 or control immunoglobulin (Ig)G were injected to the spondyloarthritis mice.The arthritis scores were analyzed by t test.Peripheral and axial joints disease development was assessed by Micro-CT and histology.Results Proteoglycan immunized mice began to develop peripheral arthritis in the 8th week.The peripheral arthritis score reached the peak (10.5±1.5) in the 11th week,with the inflammation and spur formation of the ankle and knee joint.We found infiltration of inflammation cells in intervertebral discs of the lumbar vertebrae and the caudal vertebrae.Chondrocyte proliferation couldbe seen in the meniscus of knee and lumbar intervertebral discs.In the 18th week,the intervertebral discsof thoracic vertebrae and the cervical vertebrae were also damaged.Abundant chondrocytesgathered in the intervertebra] discs.The inflammation and new bone for-marion of peripheral and axial joints were more severe in control IgG group than 093G9 group.The peripheral arthritis score in the 093G9 group decreased significantly after 2 treatments,[(2.8±1.3) vs (4.2±2.1),t=2.516,P＜0.05].The difference in arthritis scores between the two groups was the most significant after 8.treatments,[(2.0±2.0)vs (5.3±2.0),t=4.082,P＜0.01].Conclusion The mouse model of spondyloarthritissimulates human spondyloarthritis,including inflammation and new bone formation in p()gheral and axial joints.CCN1 monoclonal antibody can improve the inflammation and new bone formation inspondyloarthritis mouse model.
