Investigation on endometrium from menstrual blood as a source of non-invasive tissue
10.3760/cma.j.issn.0529-567x.2019.08.005
- VernacularTitle:月经血子宫内膜作为无创取材组织来源的研究
- Author:
Shan SHU
1
;
Qingbo FAN
;
Jinghe LANG
Author Information
1. 中国医学科学院北京协和医学院北京协和医院妇产科 100730
- Keywords:
Menstruation;
Endometrium;
Specimen handling
- From:
Chinese Journal of Obstetrics and Gynecology
2019;54(8):527-533
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility of a non-invasive sampling method by collecting menstrual blood and obtaining endometrium for further research in menstruation-related diseases.Methods On the second day of menstruation,menstrual blood was collected with menstrual cups for 4 hours,and the menstrual endometrium was filtered through a metal screen for weighing,cryopreserved,immunohistochemical staining and cell culture.Results The collection process was painless and non-invasive.In the control group,the menstrual volume was (9.1±0.7) ml,and the endometrial tissue weight was (91.0+ 14.7) g.In the endometriosis group,the menstrual volume was (9.6± 1.9) ml (P=0.022),and the endometrial tissue weight was (134.7±43.9) g (P=0.057).Endometrial cell culture was successful in all patients and should not be contaminated.The growth curve was a finite cell line type.The expression of cytokeratin 19 and vimentin in menstrual endometrium and cells were positive.Conclusions By collecting menstrual blood and filtering endometrial tissue,it is an ideal non-invasive sampling method.In combination with advanced experimental technology,menstrual endometrium make further researches of endometriosis,endometrial lesions or other menstruation-related diseases possible.
