Expression and potential role of ajuba LIM protein in oral squamous cell carcinoma
10.3969/j.issn.1000-8179.2019.14.512
- VernacularTitle:AJUBA在口腔鳞癌中的表达和意义及其相关机制
- Author:
Xiaofeng YAO
1
;
Rui JIN
;
Yingjie TAO
;
Wenyu GUO
Author Information
1. 天津医科大学肿瘤医院颌面耳鼻喉肿瘤科
- Keywords:
oral squamous cell carcinoma (OSCC);
ajuba LIM protein (AJUBA);
Snail;
proliferation;
invasion
- From:
Chinese Journal of Clinical Oncology
2019;46(14):723-727
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To assess the expression of ajuba LIM protein (AJUBA) in oral squamous cell carcinoma (OSCC) and to determine its role in cell proliferation, migration, and invasion in OSCC. Methods: The expression of AJUBA at mRNA and protein levels in OSCC was determined by quantitative polymerase chain reaction (qPCR) and immunohistochemical approaches, respectively. This was fol-lowed by analysis of the correlations between AJUBA levels and clinicopathological features of OSCC. The effects of AJUBA on cell pro-liferation, migration, and invasion in OSCC were assessed by MTT, wound healing, and transwell migration assays, respectively. West-ern blot assays were performed to check for the potential regulation of the Snail/E-cadherin pathway by AJUBA. Results: The expres-sion of AJUBA was significantly higher in OSCC tissues compared to that in adjacent normal tissues and correlated with T stage, cell dif-ferentiation, lymph node metastasis, and recurrence in OSCC. Elevated AJUBA levels indicated poor prognosis in patients with OSCC. Depletion of AJUBA impaired cell proliferation, migration, and invasion abilities of OSCC cells. Data from Western blot assays showed that AJUBA facilitated the expression of Snail but inhibited that of E-cadherin. Conclusions: AJUBA is overexpressed in OSCC and may influence cell proliferation and invasion in OSCC by modulating the Snail/E-cadherin pathway.
