Tumor Establishment Features of Orthotopic Murine Bladder Cancer Models.
10.4111/kju.2012.53.6.396
- Author:
Jin Seok LEE
1
;
Min Ho BAE
;
Sung Ho CHOI
;
Sang Hun LEE
;
Young Sam CHO
;
Heung Jae PARK
;
Chil Hun KWON
;
Kwan Joong JOO
Author Information
1. Department of Urology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea. urojoo@dreamwiz.com
- Publication Type:Original Article
- Keywords:
Animal models;
Intravesical administration;
Urinary bladder neoplasms
- MeSH:
Administration, Intravesical;
Animals;
Electrocoagulation;
Mice;
Models, Animal;
Urinary Bladder;
Urinary Bladder Neoplasms
- From:Korean Journal of Urology
2012;53(6):396-400
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Animal tumor models are important for the evaluation of novel therapeutic modalities. Since the initial report of an orthotopic bladder tumor model, several modifications have been proposed to improve the tumor take rate. Here we compared the HCl-pretreated and electrocauterization-pretreated orthotopic murine bladder tumor models. MATERIALS AND METHODS: MBT-2 murine bladder cancer cells were transurethrally implanted in the bladder of syngeneic C3H/He mice. The mice were divided into three groups according to pretreatment methods (electrocautery, HCl, and control group) and were subjected to pretreatment before instillation of MBT-2 tumor cells into the bladder. Mice were sacrificed on day 21, and bladders were harvested, weighed, and examined histopathologically. RESULTS: The tumor take rate of the control, electrocautery, and HCl groups was 0%, 54%, and 100%, respectively. The tumor take rate of the HCl group was significantly higher than that of the control group (p<0.01) and the electrocautery group (p=0.01). Pathologic reports revealed that all established bladder tumors were high-grade papillary urothelial carcinomas. CONCLUSIONS: The HCl pretreatment model was a preferable murine bladder tumor model for evaluating further therapeutic interventions.
