Study on improvement of quality standard for Kechuan Liuwei oral liquid
10.3969/j.issn.1006-0111.2019.01.013
- VernacularTitle:咳喘六味合剂质量标准提高研究
- Author:
Fanping YANG
1
;
Guowen LI
;
Yan XI
Author Information
1. 上海中医药大学附属普陀医院药物临床试验机构办公室
- Keywords:
Kechuan Liuwei oral liquid;
quality standard;
TLC
- From:
Journal of Pharmaceutical Practice
2019;37(1):55-58,85
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve the quality control standard of the hospital preparation Kechuan Liuwei oral liquid in Longhua Hospital.Methods TLC was used for qualitative identification of Scutellaria baicalensis Georgi and Asarum sieboldi Mig in the Kechuan Liuwei oral liquid.Ephedrine and Baicalin content in ephedra and Scutellaria were determined by HPLC with Welch-C18column (4.6mm×250mm, 5μm).Acetonitrile-0.1％phosphoric acid solution (4∶96) and methanol-0.1％phosphoric acid solution (47∶53) were used as mobile phase.The detection wavelengths were 206nm and 278nm respectively.The flow rate was 1.0ml/min.Results The TLC spots of Scutellaria baicalensis Georgi and asarum were clear without interference of the negative control.The linear range of Ephedrine hydrochloride was within 12.04-301.00μg/ml (r=0.999 9).The average recovery was 101.7％ (RSD=1.5％).The linear range of Pseudoephedrine hydrochloride was within 7.98-199.40μg/ml (r=0.999 9).The average recovery was 101.6％ (RSD=2.4％).The linear range of Baicalin was within 5.18-129.50μg/ml (r=0.999 9).The average recovery was 101.0％ (RSD=0.3％).Conclusion The qualitative identification and the active ingredient assay method established in this experiment were simple and feasible.Those methods can be used as the quality control standard for Kechuan Liuwei oral liquid.
