Study on fingerprint of Hupulus lupulus by HPLC
10.3969/j.issn.1006-0111.2019.01.010
- VernacularTitle:啤酒花HPLC指纹图谱研究
- Author:
Zhimin DONG
1
;
Yueming XU
;
Jun WEN
;
Yiping JIANG
;
Luping QIN
;
Hailiang XIN
Author Information
1. 上海师范大学生命与环境科学学院
- Keywords:
Hupulus lupulus;
HPLC;
fingerprint
- From:
Journal of Pharmaceutical Practice
2019;37(1):42-45,54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish fingerprint of Hupulus lupulus by HPLC.Methods Diamonsil C18column (250mm×4.6mm, 5μm) was used.The mobile phase consisted of acetonitrile and 0.1％phosphoric acid, in gradient elution mode, detection wavelength was 358nm, column pressure at 10.2MPa, column temperature was 30℃, injection volume of 10μl and flow rate was 1.0ml/min.Results A common mode of HPLC fingerprint of Hupulus lupulus was established.19common peaks were calibrated, xanthohumol, cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone for identification.Among the 26batches of Hupulus lupulus, 5batches of herbs had similarities of less than or equal 0.9, which were all wild varieties.Conclusion The method was fast, effective, accurate and reliably to distinguish the cultivation and wildness Hupulus lupulus, which provided reference for quality control of Hupulus lupulus.
