Determination of rutaecarpine in Fuzhengpingxiao capsule by HPLC method
10.3969/j.issn.1006-0111.2018.03.018
- VernacularTitle:HPLC法测定扶正平消胶囊中吴茱萸次碱的含量研究
- Author:
Shengjian LI
1
;
Xian QIAN
;
Jin ZHOU
;
Lei LÜ
;
Guoqing ZHANG
;
Liang ZHAO
Author Information
1. 上海东方肝胆外科医院药材科
- Keywords:
HPLC;
rutaecarpine;
Fuzhengpingxiao capsule;
quality standard
- From:
Journal of Pharmaceutical Practice
2018;36(3):274-276
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a new method to determine the contents of rutaecarpine in Fuzhengpingxiao capsule by HPLC method.Methods Samples were handled by ethanol and extraction with ethyl acetate.The separation was achieved on an Agilent TC-C18column using a mobile phase system of acetonitrile-water(2% Tetrahydrofuran and 0.2 % formic acid)at a flow rate of 1.0 ml/min.The temperature of column was 40 ℃ and the detection wavelength was 240 nm.Results The cali-bration curves of rutaecarpine showed good linearity in the ranges of 1.18-118 μg/ml,r=0.999 9.The results of intra-day and inter-day precisions were both within 2%,the average additional recovery rate was 94.20%.Conclusion The HPLC method was accurate,specific,sensitive and reproducible,which could be used for quality control of rutaecarpine in the preparation of Fuzhengpingxiao capsule.
