Research on quality control of Huoguwan
10.3969/j.issn.1006-0111.2018.02.012
- VernacularTitle:活骨丸质量标准研究
- Author:
Shengying YE
1
;
Qi GUO
;
Hong LEI
;
Ying YANG
Author Information
1. 解放军254医院药剂科
- Keywords:
Huoguwan;
Renshenzaogan Rg1;
Renshenzaogan Rb1;
Sanqizaogan R1;
HPLC;
TLC;
quality control
- From:
Journal of Pharmaceutical Practice
2018;36(2):156-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality control method for Huoguwan.Methods TLC was applied to detect the component of Danshen,Dihuang,Danggui,Chuanxiong,Sanqi,Tubiechong,and Zhicaowu in this compound preparation. HPLC was used to measure the concentration of Renshenzaogan Rg 1,Rb1and Sanqizaogan R1.Unitary C18analytical column (4.6 mm×250 mm,5 μm)was used with acetonitrile-water as the mobile phase(Flow rate:1.0 ml/min,column temperature:30 ℃,wavelength:203 nm).Results Each component showed distinct spots on TLC without interference.There are good linear relationship for Sanqizhaogan R1in the range of 39.92-399.2 μg/ml,Renshenzhaogan Rb1in 84.28-842.8 μg/ml and Renshenzhaogan Rg1in 135.86-1 358.6 μg/ml.The recovery was 102.35%,103.84% and 102.97% respectively.Conclusion The above described procedure was accurate and reproducible,which can be used as a method for the quality control of Huoguwan.
