Transient receptor potential type vanilloid 1 regulates EGFR related functions in pancreatic cancer cells

VernacularTitle:胰腺癌细胞中TRPV1受体参与EGFR介导的细胞功能研究
Author: Jin HUANG ¹ ; Jingxue LIU ; Ying ZENG ; Fang YUAN
Author Information

1. 上海中医药大学附属岳阳中西医结合医院药剂科
Keywords: transient receptor potential vanilloid 1; EGFR; pancreatic cancer cells; cell proliferation
From: Journal of Pharmaceutical Practice 2018;36(2):126-130
CountryChina
Language:Chinese
Abstract: Objective To evaluate the regulation of the TRPV1 receptor on the expression of EGFR protein and the effects on related cell functions.Methods Human pancreatic cancer cells PANC-1 were cultured and treated by over-expression and siRNA.Cell proliferations were detected by CCK-8 experiment.RT-PCR test was used to detect the expression of onco-genes Akt2 and K-ras.Results EGFR protein was down-regulated by TRPV1 over-expression or by agonist activation in pan-creatic cancer cells.EGFR protein was increased by the interference of TRPV 1 expression.The proliferation rate of pancreatic cancer cells was decreased and Akt2,K-ras were significantly inhibited by TRPV1 over-expressing.Conclusion The expres-sion of TRPV1 in pancreatic cancer cells regulated the EGFR protein content.The cell proliferation and oncogene expression were inhibited by TRPV1 over-expressing.