Determination of paeoniflorin in Chinese traditional herbal preparation Fuzheng-pingxiao capsule using HPLC method
10.3969/j.issn.1006-0111.2018.01.014
- VernacularTitle:HPLC法测定扶正平消胶囊中芍药苷的含量
- Author:
Zhenhe FU
1
;
Liang ZHAO
;
Jin ZHOU
;
Xinxia WANG
;
Guoqing ZHANG
;
Yufeng HUANG
Author Information
1. 第二军医大学附属东方肝胆外科医院药材科
- Keywords:
HPLC;
FuzhengPingxiao capsule;
paeoniflorin;
determination
- From:
Journal of Pharmaceutical Practice
2018;36(1):68-70,79
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of paeoniflorin in Fuzhengpingxiao capsule by HPLC method .Methods The content of paeoniflorin were determined by HPLC ,and the column was an Agilent Zorbax SB-C18 column (150 mm × 4 .6 mm ,5 μm);The mobile phase was acetonitrile and 5 mmol/L sodium dihydrogen phosphate (10:90) ,and the flow rate was set at 1 ml/min .Column temperature was 25°C ,detection wavelength was 230 nm ,injection volume was 10 μl ,and running time was 25 min .Results Paeoniflorin was linear (r=0 .9999) in the concentration of 25 .0-500 .0 μg/ml ,the linear equation was Y=12 .65 X +43 .54 ,and the average recovery was 92 .69％ with RSD value was 1 .77％ .Conclusion The method was easy to operate ,reliable and reproducible ,which could be used in the determination of paeoniflorin in FuzhengPingxiao capsule .
