Analysis of flavonoids contents of hops
10.3969/j.issn.1006-0111.2018.01.013
- VernacularTitle:啤酒花中黄酮类成分的含量分析
- Author:
Changhui LI
1
;
Yueming XU
;
Qiaoyan ZHANG
;
Ting HAN
;
Hailiang XIN
Author Information
1. 山东中医药大学药学院
- Keywords:
Humulus lupulus L .;
total flavonoids;
xanthohumol
- From:
Journal of Pharmaceutical Practice
2018;36(1):64-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the contents of total flavonoids and xanthohumol in hop from 29 different countries and regions .Methods Rutin colorimetric method was used to determine the content of total flavonoids .HPLC-UV method was established for the determination of xanthohumol in hops .HPLC method was performed by Dikma Technologies Diamonsil C18 (250 mm × 4 .6 mm ,5 μm ) column with mobile phase acetonitrile-1％ glacial acetic acid solution at the flow rate of 1 .0 ml/min .The column temperature was 25 ℃ .The detection wavelength was 370 nm .Results The equation of linear re-gression of total flavonoids was A=30 .345C+0 .0168 ,r=0 .9999 .The equation of linear regression of xanthohumol was A=55446 C+9040 .5 ,r=0 .9999 .Their linear ranges were respectively 20 .2-404 .0 μg/ml ,2 .152-43 .040 μg/ml ,which indica-ted a good linear relationship .The RSDs of precision and repeatability were less than 2％ .The average recoveries of flavonoids and xanthohumol were respectively 102 .71％ and 100 .21％ .Conclusion The contents of total flavonoids and xanthohumol in different hops varieties are significantly different and the import hops was better than the domestic hops in this study .
