Studies on the binding properties of Zn2+ ion to quercetin and the antioxidant ac-tivi ties
10.3969/j.issn.1006-0111.2018.01.010
- VernacularTitle:槲皮素与锌离子结合性质及抗氧化活性研究
- Author:
Lijuan QIU
1
;
Xin MENG
;
Shengshu HOU
;
Genjin YANG
;
Weiqing ZHONG
Author Information
1. 第二军医大学 基础医学院
- Keywords:
quercetin;
Zn2+ ion;
binding constant;
antioxidant activity
- From:
Journal of Pharmaceutical Practice
2018;36(1):50-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-oxidation properties of Zn2+-quercetin complexes .Methods The binding stoi-chiometric ratios and the binding constant of Zn2+ to quercetin were measured with UV-Vis spectroscopy .The binding sites on quercetin were determined via1 H NMR spectroscopy .The antioxidant activities of Zn2+-quercetin complexes were compared to quercetin by scavenging DPPH radical method .The binding ratio of Zn2+ to quercetin depends on neutralization of phenol groups of quercetin .Results The binding ratio of Zn2+ to quercetin is 1:2 when less than two OH groups were deprotonated . The binding ratio is 1:1 when more than three OH groups were deprotonated .The apparent binding constant is 2 .42 × 106 M -1 for the 1:1 Zn2+-quercetin complex .The 3′-OH and 4′-OH of quercetin are involved in the Zn2+ binding .The scavenging DPPH radical activity of Zn2+-quercetin complex is 2 .3 times of quercetin .Conclusion These results provide a new insight to expand applications of this traditional Chinese medicine .
