Simultaneous determination of dexamethasone acetate , camphor and phenol in compound cream by HPLC method
10.3969/j.issn.1006-0111.2017.06.017
- VernacularTitle:HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚的含量
- Author:
Yali WANG
1
;
Xiandong PENG
;
Weiyi HAO
;
Dan WANG
;
Tingdong GUO
;
Zhili TANG
Author Information
1. 南充市中心医院药学部
- Keywords:
HPLC;
cream;
dexamethasone acetate;
camphor;
phenol;
determination
- From:
Journal of Pharmaceutical Practice
2017;35(6):551-553,576
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for simultaneously determining the content of dexamethasone ace-tate ,camphor and phenol in compound cream .Methods The separation was performed on a SHIADZU-GL Inertsil? ODS-3 RP C18 analytical column with the mobile phase consisting of methanol-water (60:40) .The flow rate was 1 .0 ml/min .The detection wave length was 285 nm and the column temperature was 40 ℃ .Results Dexamethasone acetate ,camphor and phe-nol showed good linearity (r> 0 .9995 , n= 7) within the concentration range of 4 .024-40 .24 ,101 .7-2033 and 10 .38-425 .2 μg/ml ,respectively . The average recovery of dexamethasone acetate ,camphor and phenol was 101 .2% (RSD was 0.56% ) ,99 .89% (RSD was 0 .72% ) ,100 .2% (RSD was 0 .97% ) ,respectively .Moreover ,the RSDs were less than 1 .5% in the repeated tests .Conclusion The method was simple ,quick and accurate .It is suitable for the quality control of dexametha-sone acetate camphor and phenol cream .