Research on the determination of total flavonoids in compound Yinchen mixture
10.3969/j.issn.1006-0111.2017.06.016
- VernacularTitle:复方茵陈合剂总黄酮测定方法研究
- Author:
Xiaoling LIU
1
;
Xiaohui CAI
;
Xiaoxia NI
;
Qingfen WANG
Author Information
1. 解放军175医院/厦门大学附属东南医院
- Keywords:
UV spectrophotometry;
compound Yinchen mixture;
total flavonoids;
content determination
- From:
Journal of Pharmaceutical Practice
2017;35(6):547-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of the total flavonoids content in compound Yinchen mixture by UV spectrophotometry .Methods Using rutin as comparison ,three coloration methods were studied to find the op-timal assay method .Results The sample was detected at 508 nm wavelength by NaNO2-Al(NO3 )3-NaOH reaction with rutin as reference .The rutin content had a liner relationship in the range of 0 .0125-0 .0626 g/L (n=9 ,r=0 .9999) ,and the aver-age recovery rate was 99 .49％ with RSD of 0 .84％ .Conclusion The NaNO2-Al(NO3 )3-NaOH coloration method is proved to be simple ,quick ,stable and reliable for the determination of total flavonoids in compound Yinchen mixture .
