Study on quality standard of compound Heishen granules
10.3969/j.issn.1006-0111.2017.06.015
- VernacularTitle:复方黑参颗粒质量标准研究
- Author:
Guixiang ZHONG
1
;
Jia YAN
;
Qiaoting TANG
;
Hongtao SONG
;
Xin ZHOU
Author Information
1. 福州总医院
- Keywords:
compound Heishen granules;
cinnamic acid;
tectoridin;
irisflorentin;
HPLC;
TLC
- From:
Journal of Pharmaceutical Practice
2017;35(6):543-546
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a quality standard for compound Heishen granules .Methods Scrophulariae Radix and Belamcandae Rhizoma were identified by TLC .HPLC was used to determine the content of cinnamic acid ,tectoridin and irisflo-rentin .The HPLC was performed on a column of Kromasil-C18 (150 mm × 4 .6 mm ,5 μm)with a mobile phase of acetonitrile (A)-0 .1％ hydrochloric acid (B)at a temperature 30 ℃ .The detection wavelength was set at 270 nm and the flow rate at 1 ml/min .Results The TLC method had good specificity without interference from negative control .The calibration curve showed a good linear relationship within the range of 16 .22-113 .57μg/ml for cinnamic acid(r=0 .9998) ,48 .19-337 .34μg /ml for tectoridin(r= 0.9998)and 16.40-114.80 μg/ml for irisflorentin(r= 0.9999) .The average recoveries were 99.23％ , 98.82％ ,99.17％ .Conclusion The established method is rapid ,accurate and reproducible .It can be used in the quality control of compound Heishen granules .
