Content variation of four flavonoid glycosides in Hippophae rhamnoides L .leav-es before and after fermentation assayed by RP-HPLC
10.3969/j.issn.1006-0111.2017.06.011
- VernacularTitle:RP-HPLC法测定沙棘叶发酵前后4种黄酮苷类成分含量的变化
- Author:
Ruifang DONG
1
;
Zheng WANG
;
Ling CHEN
;
Zhiheng SHU
;
Ting HAN
;
Luping QIN
Author Information
1. 上海师范大学生命与环境科学学院
- Keywords:
RP-HPLC;
Hippophae rhamnoides L .leave;
fermentation;
flavonoid glycosides
- From:
Journal of Pharmaceutical Practice
2017;35(6):526-529
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the changes of four flavonoid glycosides in the leaves of Hippophae rhamnoides L . before and after fermentation .Methods The water extract of Hippophae rhamnoides L .leaves and its fermented tea were con-centrated and desiccated .The dry extracts were dissolved in 70％ ethanol .The chromatographic separation was performed with RP-HPLC method on an Extend-C18 column (4 .6 mm × 250 mm ,5 μm) .Acetonitrile-0 .1％ formic acid was selected as mobile phase at the flow rate of 1 .0 ml/min .The detection wavelength was 356 nm and the column temperature was 30 ℃ .Results The rutin content was high in the leaves of Hippophae rhamnoides L .After fermentation ,isoquercitrin content was increased , while the contents of rutin and narcissoside were reduced and isorhamnetin-3-O-glucoside stayed unchanged .There was a good linear relationship between the concentration and peak areas of the four compounds (r>0 .9997) .The average recoveries were between 96％-103％ .Conclusion This established method is rapid and reliable ,which can be used for the quality control of Hippophae rhamnoides L .leaves and its fermented tea .
