Study on the technology for extracting lignans in schisand ra chinensis (Turcz ) Baill by AB-8 macroporous resin
10.3969/j.issn.1006-0111.2017.06.010
- VernacularTitle:AB-8大孔树脂纯化五味子木脂素工艺研究
- Author:
Yiping JIANG
1
;
Liming XUE
;
Hailiang XIN
;
Qiaoyan ZHANG
;
Luping QIN
Author Information
1. 第二军医大学药学院生药学教研室
- Keywords:
schisandra chinensis (Turcz) Baill;
lignans;
AB-8 macroporous resin
- From:
Journal of Pharmaceutical Practice
2017;35(6):520-525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the conditions for isolating and purifying lignans in schisandra chinensis (Turcz) Baill by AB-8 type of macroporous adsorption resin .Methods The content of Schizandrol ,Deoxyschizandrin ,r-Schisandrin was deter-mined by HPLC .The optimum separation and purification process of lignans from schisandra chinensis (Turcz) Baill with AB-8 macroporous adsorption resin were identified by static and dynamic adsorption ,desorption tests .The sample volume ,concen-tration ,eluting velocity ,and ethanol concentration were investigated .Results The optimum conditions for isolating and purif-ying lignans in schisandra chinensis (Turcz) Baill by AB-8 macroporous adsorption resin were as follows :dosage of sample liq-uid was 1BV ,concentration of sample solution was 9 .159-16 .523 mg/ml ,ratio of diameter to height of resin column 1:5 ,ad-soption flow rate was 2 .5 BV/h ,eluted by 5 BV 30％ ethanol ,followed by 10BV 95％ ethanol .The transfer rate of lignans was 77 .07％ with 22 .06％ of total lignan content .Conclusion AB-8 macroporous adsorptive resin can effectively isolate and purify lignans from schisandra chinensis (Turcz) Baill .This low cost process is easy to operate and with high industrial pro-duction value .
