Effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO

VernacularTitle:氟尿嘧啶对结直肠癌RKO细胞SHP2基因表达的影响
Author: Jie WANG ¹ ; Yan HUANG ; Chuan ZHANG ; Xingyuan MA ; Wenjun CHANG ; Yiming LU
Author Information

1. 第二军医大学药学院生化药学教研室
Keywords: 5-FU; SHP2; colorectal cancer cells; immunofluorescence
From: Journal of Pharmaceutical Practice 2017;35(6):512-515
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of 5-FU on the expression of SHP2 gene in colorectal cancer cells RKO . Methods The human colorectal cancer cell line RKO of logarithmic growth phase was cultured 48 h with 5-FU .The expression of SHP2 was observed by immunofluorescence and Western blotting ;siSHP2 which specifically inhibits the expression of SHP2 was transfected into RKO cells and cultured 48 h with 5-FU .The cell absorbance (A) values were measured with CCK8 and apoptosis was determined by flow cytometry .The sensitivity of RKO to 5-FU and the effect of 5-FU on apoptosis of RKO cells were observed .Results The expression of nuclear protein of SHP2 was improved remarkably after colorectal cancer cell RKO was cultured 48 h with 5-FU .The sensitivity of RKO cells to 5-FU and the cell apoptosis rate induced by 5-FU were decreased after siSHP2 transfection .Conclusion 5-FU exerts anti-cancer activity possibly due to its promoting the apoptosis of RKO cells by influencing the expression of SHP2 .