Determination of sinoporphyrin sodium in tumor-bearing mouse plasma by HPLC method
10.3969/j.issn.1006-0111.2017.06.007
- VernacularTitle:HPLC法测定荷瘤小鼠血浆中华卟啉钠含量及药动学研究
- Author:
Bing ZHU
1
;
Lihong GAO
;
Naping ZHAO
;
Shanxin LI
;
Li ZHANG
Author Information
1. 第二军医大学附属长海医院药学部
- Keywords:
sinoporphyrin sodium;
HPLC;
pharmacokinetics
- From:
Journal of Pharmaceutical Practice
2017;35(6):508-511,558
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the assay of sinoporphyrin sodium (DVDMS) in tumor-bearing mouse plasma and to study its pharmacokinetics .Methods The column was Waters XBridge C18 (3 .0 mm × 100 mm ,3 .5μm) . Gradient elution was applied with mobile phase A as the mixture of acetonitrile-methanol (20:80) and B as the aqueous solu-tion of 1％ acetic acid and 0 .1％ triethylamine at flow rate 0 .7 ml/min .The detection wavelength was 380 nm .DVDMS was administrated to tumor-bearing mice by tail vein injection .The blood samples were collected at designated time and centrifuged for plasma .DVDMS in plasma samples were extracted by protein precipitation and analyzed by the HPLC method mentioned a-bove .Pharmacokinetic parameters were calculated by DAS 2 .0 with statistical moment analysis .Results DVDMS showed good linearity within the ranges of 70 .8-14160 ng/ml (r=0 .9998) .The main pharmacokinetic parameters were calculated as follows :cmax = (24127 .59 ± 1415 .23) ng/ml ,tmax =0 .083 h ,t1/2 = (9 .59 ± 1 .25) h ,MRT0-∞ = (11 .77 ± 1 .73) h ,AUC0-∞ =(34775 .83 ± 6185 .43) h · ng/ml .Conclusion This HPLC method is sensitive ,rapid and accurate ,which can be used for a-nalysis and research of DVDMS in plasma samples of tumor-bearing mice .
