Pharmacokinetics of paclitaxel loaded nanoparticles made with hydrophobically modified Rhizoma Bletillae polysaccharide in rats
10.3969/j.issn.1006-0111.2017.06.006
- VernacularTitle:疏水改性白及多糖自组装载紫杉醇纳米粒子在大鼠体内药动学研究
- Author:
Qi ZHANG
1
;
Wencui ZHAO
;
Yang LIU
;
Gongliang ZHANG
;
Nian CHENG
;
Jihua HOU
Author Information
1. 江西中医药大学
- Keywords:
Rhizoma Bletillae polysaccharide;
paclitaxel;
nanoparticles;
pharmacokinetics
- From:
Journal of Pharmaceutical Practice
2017;35(6):504-507
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple and sensitive method for the determination of plasma drug concentration in rats after intragastrical and intravenous administration of paclitaxel loaded nanoparticles and to evaluate its pharmacokinetic characteristics .Methods AgilentTC-C18 column (250 mm × 4 .6 mm ,5 μm) was used for HPLC at the flow rate 1 ml/min with ketoconazole as internal standard and methanol acetonitrile water (45:20:35) as the mobile phase .The statistical mo-ment method was applied to calculate the pharmacokinetic parameters and to evaluate pharmacokinetic characteristics .Results There was a good liner relationship (r=0 .9997) when rat blood concentration of Paclitaxel ranged from 0 .5 to 20μg /ml .The accuracy and precision were in line with the requirements of biological sample analysis .The t1/2 were 3.86 ,3.76 ,3.35 and 2 .62 h after intravenous injection of three lab-made paclitaxel nanoparticles and paclitaxel solution via rat tail vein .The t1/2 were 5 .28 and 3 .72 h respectively after intragastrical administration of lab-made paclitaxel nanoparticles and paclitaxel suspen-sion .Conclusion This method can be used for the pharmacokinetic study of paclitaxel nanoparticles in rats .The paclitaxel loaded nanoparticles exhibited significantly longer in vivo retention time than paclitaxel injection and paclitaxel suspension .
