One step assay for five components in Xianlinggubao capsule by HPLC method
10.3969/j.issn.1006-0111.2017.05.014
- VernacularTitle:HPLC法测定仙灵骨葆胶囊中5种成分的含量
- Author:
Zongliang CHEN
1
;
Xiaoxia LV
;
Guiqian CHEN
;
Jie CHEN
Author Information
1. 金华市食品药品检验检测研究院
- Keywords:
HPLC;
Xianlinggubao capsule;
Icariine;
Epimedin C;
asperosaponin Ⅵ;
psoralen;
angelicin
- From:
Journal of Pharmaceutical Practice
2017;35(5):444-446,452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assay Icariine, Epimedin C, asperosaponin Ⅵ, psoralen and angelicin in Xianlinggubao capsules via multi-wavelength HPLC method.Methods Separation was carried out on Welch Ultimate○R XB-C18 column.The mobile phase was acetonitrile-water system and a linear gradient elution was used.The column temperature was 30 ℃.The detection wavelength for Icariine, Epimedin C,asperosaponin Ⅵ was set at 212 nm, psoralen and angelicin at 246 nm.Results Five components reached baseline separation, the linearity was good when sample size was in the range of 0.008 2-0.328 μg for Icariine(r=0.999 5), 0.055 6-2.224 μg for Epimedin C (r=0.999 6), 0.144 1-5.764 μg for asperosaponin Ⅵ(r=0.999 6), 0.005 4-0.215 2 μg for psoralen(r=0.998 0), 0.006 6-0.265 6 μg for angelicin(r=0.998 5).The average recoveries were 97.59％, 98.58％, 98.11％, 97.86％, 98.22％ respectively.The RSDs of recovery were all less than 2.0％.Conclusion This method is simple, accurate, with good separation, high sensitivity for the assay of multiple components in Xianlinggubao capsule.
