Determination of total anthraquinones in Xiaofan lotion by HPLC method
10.3969/j.issn.1006-0111.2017.05.013
- VernacularTitle:HPLC法测定硝矾洗剂中大黄总蒽醌的含量
- Author:
Juan HUANG
1
;
Qinglian ZHANG
;
Fengjuan PI
;
Shibo ZHANG
;
Ling ZHAO
;
Hao LIU
Author Information
1. 泸州市中医医院药剂科
- Keywords:
Xiaofan lotion;
HPLC;
total anthraquinone
- From:
Journal of Pharmaceutical Practice
2017;35(5):441-443
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a method for the determination of total anthraquinones in Xiaofan lotion by HPLC.Methods Rhubarb anthraquinones were simultaneously separated and assayed on a Kromasil C18 column(250 mm×4.6 mm,5 μm) with the mobile phase of 0.1％phosphoric acid-methanol (15∶85) at 30 ℃.The detection wavelength was 207 nm.Results The linear relationship is good for Aloe-emodin and other five standard ingredients.The average recovery was 99.45％, RSD 1.68％.Conclusion The method is simple, rapid and accurate.It is suitable for determination of total anthraquinones in Xiaofan lotion.
