Rapid identification of inactivated C.albicans by surface-enhanced Raman spectroscopy

VernacularTitle:表面增强拉曼光谱法快速鉴别失活白色念珠菌
Author: Kebing WANG ¹ ; Zhihong CHEN ; Bu GUO ; Guozhong LU
Author Information

1. 解放军169医院药械科
Keywords: C.albicans; surface-enhanced Raman spectroscopy(SERS); inactivation; rapid identification
From: Journal of Pharmaceutical Practice 2017;35(5):422-426
CountryChina
Language:Chinese
Abstract: Objective To develop a rapid method for the identification of inactivated C.albicans by surface-enhanced Raman spectroscopy (SERS).Methods Live C.albicans cultures were exposed to heating, formaldehyde and fungicidal drug (amphotericin B).The corresponding SERS spectra were acquired for the investigation and comparison.Results The spectra acquired with three different inactivation methods exhibited similar features of dead C.albicans, which showed significant difference from the spectra of the live culture.Conclusion This SERS method can identify the inactivated C.albicans rapidly.Hopefully it will provide a convenient tool for quick identification of other inactivated pathogenic microorganisms.