Determination of madecassoside and asiaticoside in Centella asiatica formula granules by HPLC method
10.3969/j.issn.1006-0111.2017.04.018
- VernacularTitle:HPLC法测定积雪草配方颗粒中羟基积雪草苷和积雪草苷含量
- Author:
Mei YANG
1
;
Junyuan LI
;
Jiabin WU
;
Qiuping YU
;
Minling WEI
;
Hongyan WEI
Author Information
1. 培力(南宁)药业有限公司
- Keywords:
Centella asiatica;
formula granules;
content determination;
madecassoside;
asiaticoside;
HPLC
- From:
Journal of Pharmaceutical Practice
2017;35(4):359-361
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of madecassoside and asiaticoside in Centella asiatica formula granules.Methods Chromatographic separation was performed on Ultimate AQ-C18 column(4.6 mm×250 mm,5 μm) with mobile phase of acetonitrile(A)-2 mmol/L β-cyclodextrin(0~30 min:21% A→23% A;30~60 min:23% A→25% A).The flow rate was set at 1.0 ml/min, the column temperature at 30 ℃ and detection wavelength at 205 nm.Results Madecassoside and asiaticoside showed good linearity (r>0.9995) in the ranges of 0.187 7~3.754 μg and 0.184 3~3.686 μg respectively.The specificity, repeatability, precision,recovery and stability were satisfied to the method validation requirements of China Pharmacopoeia.Conclusion The method can determine madecassoside and asiaticoside in Centella asiatica formula granules.
