The quality standard research for YI SHI MINGMU granules by HPLC method
10.3969/j.issn.1006-0111.2017.04.017
- VernacularTitle:益视明目颗粒质量标准研究
- Author:
Jing MIAO
1
;
Xiaodong TONG
Author Information
1. 南通市中医院
- Keywords:
YI SHI MINGMU granules;
quality standard;
Tanshinol sodium
- From:
Journal of Pharmaceutical Practice
2017;35(4):355-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the quality standard for YI SHI MINGMU granules.Methods TLC method was used to identify Lycium barbarum L and Salvia miltiorrhiza.HPLC method was used to quantitatively analyze the concentration of Tanshinol sodium.The analysis was carried out on a column of Kromasil C18(4.6 mm×150 mm,5 μm)with a mobile phase of methanol and 0.5 % acetic acid at the flow rate of 0.4 ml/min.Column temperature was 30 ℃.The detection wavelength was 280 nm.Results The linearity range of Tanshinol sodium was 2.00~60.00 μg/ml ,r2=0.999 7(n=6),with the average recoveries of 105.62%, RSD=1.60%.Conclusion This method is accurate, simple, reliable and reproducible.It can be used for the quality control of YI SHI MINGMU granules.
