Berberine hydrochloride assay in Dongbai Tonglin Heji by HPLC method
10.3969/j.issn.1006-0111.2017.04.016
- VernacularTitle:HPLC法测定冬柏通淋合剂中盐酸小檗碱的含量
- Author:
Xiaoli HOU
1
;
Zhaodan TAN
;
Kourong SHI
;
Weiying GU
;
Weicheng CHEN
Author Information
1. 上海中医药大学附属第七人民医院
- Keywords:
Dongbai Tonglin Heji;
berberine hydrochloride;
HPLC
- From:
Journal of Pharmaceutical Practice
2017;35(4):353-354,378
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of berberine hydrochloride in Dongbai Tonglin Heji.Methods The separation column of Kromasil C18(150 mm×4.6 mm,5 μm) was used.The mobile phase was acetonitrile and 0.05 mol/L NaH2PO4 solution(pH was adjusted to about 3 with phosphoric acid).The flow rate was 1.0 ml/min with the column at room temperature and the detection wavelengths at 345 nm for berberine hydrochloride.Results The linear range of berberine hydrochloride was 1.00~50.00 μg/ml(r=0.999 6).The average recoveries of berberine hydrochloride were 104.70%(RSD 1.60%, n=5).Conclusion This method is accurate, sensitive, selective and reproducible.It provides an alternative method to improve the quality control of Dongbai Tonglin Heji.
