Research on quality standard of Santeng oral solution

VernacularTitle:三藤口服液的质量标准研究
Author: Xiaoling CHEN ¹ ; Shun CHEN ; Shouhong GAO ; Chang SHU ; Tao PANG ; Wenquan LU
Author Information

1. 第二军医大学附属长征医院药学部
Keywords: Santeng oral solution; quality standard; TLC; HPLC; chlorogenic acid
From: Journal of Pharmaceutical Practice 2017;35(4):350-352,384
CountryChina
Language:Chinese
Abstract: Objective To establish the quality standard of Santeng oral solution.Method Sargentodoxa Caulis and Spatholobi Caulis were identified by thin layer chromatography.Chlorogenic acid was assayed by high performance liquid chromatography.The chromatographic column is Agilent Zorbax SB C18 (4.6 mm×250 mm, 5 μm) with a stable temperature of 35 ℃.The mobile phase in isocratic elution consists of acetonitrile and 0.1% folic acid aqueous solution with a preliminary volume ratio of 9∶91.The flow rate is 1.0 ml/min with an injection volume of 20 μl.Results Thin layer chromatography showed distinct spots of Sargentodoxa Caulis and Spatholobi Caulis with a great specificity.A regression formula Y=60.14X-6.37(r>0.999 9) was obtained with a good linearity in concentration range of 2.70~202.50 μg/ml.Conclusion A simple, stable and repeatable method was established for the quality control of Santeng oral solution.