Determination of pueratin in Jiangzhi Hugan capsule by HPLC
10.3969/j.issn.1006-0111.2017.03.016
- VernacularTitle:高效液相色谱法测定降脂护肝胶囊中葛根素的含量
- Author:
Wei ZHENG
1
;
Dongjie NI
;
Qing WEI
;
Hui XIANG
;
Lanlan HU
;
Liang SUN
Author Information
1. 解放军98医院
- Keywords:
HPLC;
Jiangzhi Hugan capsule;
pueratin;
content determination
- From:
Journal of Pharmaceutical Practice
2017;35(3):259-261
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for determination of pueratin.Methods The separation was carried out on a Waters Symmetry C18 column(4.6 mm×250 mm, 5 μm), the mobile phase was composed of acetonitrile and 1% formic acid(11∶89), the detection wavelength was set at 250 nm, the flow rate was 1.0 ml/min, the column temperature was 30 ℃ and the injection volume was 10 μl.Results The linearity was obtained over 2~40 μg/ml (r=0.999 8) for pueratin.The RSD of precision were less than 2%.The average recovery was between 98% and 103%.Conclusion This HPLC method was simple, accuracy and suitable for the quality control of Jiangzhi Hugan capsule.
