Qualitative identification and quantitative determination of Fufang Shenghua granules
10.3969/j.issn.1006-0111.2017.03.014
- VernacularTitle:复方生化颗粒的定性鉴别和含量测定研究
- Author:
Hua CHEN
1
;
Botao YU
;
Weihua JIN
;
Zhiqiang PU
;
Zonghui SONG
;
Kaihua FAN
Author Information
1. 成都军区总医院药剂科
- Keywords:
Fufang Shenghua granules;
quality standard;
TLC;
HPLC;
amarogentin;
salvianolic acid B
- From:
Journal of Pharmaceutical Practice
2017;35(3):252-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To improve quality standard of Fufang Shenghua granules.Methods TLC was used to identify chief components in the preparation, Radix et Rhizoma Glycyrrhizae and Salvia Miltiorrhiza.HPLC was applied to identify Amarogentin and to determine the content of Salvianolic acid B.Salvianolic acid B assay was performed on Agilent HC-C18(4.6 mm×250 mm, 5 μm) column with Acetonitrile-0.1% phosphoric acid (23∶77)as mobile phase.The flow rate was 1.0 ml/min.The column temperature was 30 ℃.The detection wavelength was set at 286 nm.Results The spots on TLC were fairly clear with good separation.There was no interference from the negative control samples.However, HPLC was a more accurate, reliable and objective method for qualitative identification.Salvianolic acid B showed a good linear correlation in the range of 1.56~49.92 μg/ml (r=0.999 9).The average recovery was 100.07%, RSD 1.61% (n=9).Conclusion A simple, accurate and reliable method was developed for the quality control of Fufang Shenghua granules.
