The methodology validation and content assay of total polysaccharides in Pudi Enema
10.3969/j.issn.1006-0111.2017.03.013
- VernacularTitle:蒲地灌肠液中总多糖含量测定与方法学验证
- Author:
Yihong WANG
1
;
Jinshan CHEN
;
Caiyan XU
;
Shaozhong ZHENG
Author Information
1. 解放军175医院/厦门大学附属东南医院
- Keywords:
Pudi Enema;
phenol-sulfuric acid method;
total polysaccharides;
UV spectrophotometry;
content assay
- From:
Journal of Pharmaceutical Practice
2017;35(3):248-251
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the assay method for the total polysaccharide in Pudi Enema.Methods Phenol-sulfuric acid method was used for chromogenic reaction.The content of total polysaccharide was measured by UV spectrophotometry at 488.8 nm.Results The total polysaccharides calibration curve was at the range of 0~22.635 mg/L, with regression function being Y=0.062 06 X-0.003 34(r=0.999 8).The recovery of calycosin was 98.36%(RSD=2.34%).Conclusion This method is sensitive,rapid,accurate and reliable.It can be used to assay the content of total polysaccharide in Pudi Enema.
