Cuprous oxide nanoparticles effect on the epithelial-mesenchymal transition of B16 cells
10.3969/j.issn.1006-0111.2017.03.010
- VernacularTitle:氧化亚铜纳米粒对B16细胞上皮间质转化的影响
- Author:
Xiangui LI
1
;
Jieqiong ZHAN
;
Xinfang LI
;
Lan SHE
;
Zhiqiang MA
;
Linhong SUN
;
Feng YANG
Author Information
1. 第二军医大学药学院无机化学教研室
- Keywords:
cuprous oxide nanoparticles;
invasion and metastasis of tumor;
epithelial-mesenchymal transition;
B16 cells
- From:
Journal of Pharmaceutical Practice
2017;35(3):233-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the epithelial-mesenchymal transition (EMT) effects of cuprous oxide nanoparticles (CONPs) on melanoma.Methods Cuprous oxide nanoparticles were prepared hydrothermally.The B16 cells were cultured with cuprous oxide nanoparticlesat different concentrations (5,25,50 μg/ml).The changes of the morphology of the B16 cell were observed under the inverted microscope.The effects of CONPs on B16 cell migration ability were detected through the Wound healing assay and the Transwell assay.Then cell immunofluorescence and western blotting were used to test the EMT related molecular markers, including E-cadherin, N-cadherin, Cytokeratin, and Vimentin.Results The synthesized cuprous oxide nanoparticles distribute uniformly with a diameter of 40 nm.Our study indicated that CONPs inhibited the EMT of B16 cell.A conversion process was discovered in this study.In B16 cells, CONPs inhibited B16 cell migration, promoted the expression of E-cadherin, Cytokeratin and Desmoplakin, while the expression of N-cadherin and Vimentin was repressed in protein level.Conclusion Cuprous oxide nanoparticles can significantly restrain the invasion and metastasis of melanoma cells and inhabit the EMT of B16 cells.
