Regulation and control of transcription factor Cup2 on Cu,2+ metabolism and oxidative stress in Candida albicans
10.3969/j.issn.1006-0111.2017.03.008
- VernacularTitle:转录因子Cup2对白念珠菌铜离子代谢、氧化应激调控作用的初步研究
- Author:
Jinyu ZHANG
1
;
Lihong WANG
;
Yulin QIN
;
Lulu ZHANG
;
Yuanying JIANG
;
Yongbing CAO
Author Information
1. 第二军医大学药学院新药研究中心
- Keywords:
Candida albicans;
transcription factor Cup2;
copper ion (Cu,2+);
hydrogen peroxide(H2O2);
drug susceptibility
- From:
Journal of Pharmaceutical Practice
2017;35(3):224-228
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the transcription factors of copper ion (Cu,2+) metabolism and oxidative stress by Candida albicans knocked down different transcription factors.Methods Spot assay, growth curve were used.Results The sensitivity to Cu,2+ in Cup2Δ/Δ was increasing and the growth of Cup2Δ/Δ was inhibited in 5 mmol /L Cu,2+ medium.The results showed that Cup2Δ/Δ also increased the sensitivity to H2O2, interestingly, Cu,2+and H2O2 played a synergistic antifungal effect.The tolerance of Cup2Δ/Δ and SN250 to H2O2 induced oxidative stress was increased after BCS chelating Cu,2+.In the fluconazole, miconazole and ketoconazole susceptibility experiments, Cup2Δ/Δ did not show susceptibility to azole drugs.Conclusion Knockout transcription factor Cup2, which could increase the sensitivity to Cu,2+ and H2O2in Candida albicans.Transcription factor Cup2 might be involved in the regulation and control of Candida albicansmetabolism on Cu,2+ and oxidative stress induced by H2O2, but not involved in the regulation and control of drug resistance to azole drugs.
