TLC-SERS for detection of the dyed Croci Stigma
10.3969/j.issn.1006-0111.2017.03.006
- VernacularTitle:薄层色谱-表面增强拉曼光谱法快速检测染色掺伪的西红花
- Author:
Binbin ZHANG
1
;
Yi SHI
;
Hui CHEN
;
Qingxia ZHU
;
Feng LU
Author Information
1. 第二军医大学药学院
- Keywords:
TLC;
SERS;
Croci Stigma;
dyeing adulteration;
auramine O;
new fuchsin;
tartrazine;
brilliant ponceau
- From:
Journal of Pharmaceutical Practice
2017;35(3):215-218
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a thin layer chromatography-surface-enhanced Raman scattering(TLC-SERS) method for the detection of dyed Croci Stigma.Method The dyed Croci Stigma was wetted by ethanol and pressed on TLC plate sprayed with Silver sol.The pressed zone was immediately detected by a portable Raman spectrometer.Factors including the concentration of ethanol, spraying time and amount of silver sol were investigated and optimized.Results The dyed Croci Stigma, stained by auramine O, new fuchsin, tartrazine and brilliant ponceau were successfully detected using the established method.Conclusion The combination of TLC and SERS technology provides a rapid, simple and sensitive detection method which is suitable for quick field tests.
